The Fun 🤩 Process!
I cover the basics below but, of course there is an art to urine microscopy and you should learn this from the greats so please remember to check out Twitter and follow the Legend @VelezNephHepato (Dr Juan Carlos Q Velez, Academic Nephrologist, at Ochsner in LA): his lessons are priceless! Check out and follow another stellar teacher also: The Great @jrseltzer (Dr Jay Seltzer: Chief of Nephrology at Missouri Baptist Medical Center).
- Collect 10 to 12 mL of urine: clean catch midstream urine is preferred but by all means extract catheter specimen if that is what is available.
- Centrifuge at 3000 RPM for approximately 5 to 7 minutes
- Pour off the supernatant in total: don’t worry just enough urine will cling to the sides of the tubes to resuspend your sediment!
- Suspend your sediment in the tiny bit of urine remaining above sediment. You may gently tap or flick the bottom of the tube to assist the process!
- Use a CLEAN 😊 pipette to aspirate your resuspended sediment and place one drop of same aspirated liquid gold onto your microscope slide and go on, cover it up with a coverslip!
- Examine under low power field while scanning the entire area covered by the cover slip: at this time count the number of casts you see and report her low power field (LPF)
- Switch to high power field and look for cells. The number of cells and report per high power field (HPF)
You can absolutely use stains if available and we will cover those later.